Active Intercellular Adhesion Molecule 1 (ICAM1)
CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor
- Product No.APA548Hu62
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point5.8
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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For more details, please contact local distributors! US$
For more details, please contact local distributors! US$
For more details, please contact local distributors! US$
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Intercellular Adhesion Molecule 1 (ICAM1)
Intercellular Adhesion Molecule 1 (ICAM1) is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFα) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes.As ICAM1 has the function of cell adhesion, we measure the activity of ICAM1 by the ability of the immobilized protein to support the adhesion of PMA-stimulated MOLT4 human acute lymphoblastic leukemia cells.. When 1 x 106 cells/well are added to Recombinant Human ICAM 1/CD54 coated plates (1.25 μg/mL with 100 μL/well), >10% cells will adhere after 2 hour incubation at 37 °C.The adhesion of MOLT4 after 2 hour incubation at 37℃ observed by inverted microscope was shown in Figure 1.
USAGE of the Active Intercellular Adhesion Molecule 1 (ICAM1)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Intercellular Adhesion Molecule 1 (ICAM1)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Intercellular Adhesion Molecule 1 (ICAM1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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