Active Hedgehog Homolog, Sonic (SHH)
HHG1; HLP3; HPE3; SMMCI; SHHN; SHH-N
- Product No.APB831Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point7.1
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
-
FOB
US$ 211
For more details, please contact local distributors!US$ 528
For more details, please contact local distributors! US$ 1056
For more details, please contact local distributors! US$ 3168
For more details, please contact local distributors! US$ 7920
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
SDS-PAGE
-
ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Hedgehog Homolog, Sonic (SHH)
Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. It has been reported that SHH can induce alkaline phosphatase production in C3H10T1/2 mouse embryonic fibroblast cells. To test the bioactivity of SHH, C3H10T1/2 cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of SHH for 24h and alkaline phosphatase levels in the cell supernatant were determined by ELISA. Alkaline phosphatase levels in the cell supernatant of C3H10T1/2 cells increased significantly after stimulated with SHH have shown in Figure1.
USAGE of the Active Hedgehog Homolog, Sonic (SHH)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Hedgehog Homolog, Sonic (SHH)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Hedgehog Homolog, Sonic (SHH)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
BCA Protein Quantification Kit
Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Buffer
Lentivirus Packaging Experiment Service
Adenovirus Packaging Experiment Service
Real Time PCR Experimental Service
Spike RBD Protein (S-RBD)
Protein G
Protein A