Growth Hormone (GH) is a 191-amino acid polypeptide hormone synthesized and secreted by somatotroph cells of the anterior pituitary gland. As a master regulator of somatic development, GH exerts its biological effects primarily through binding to the growth hormone receptor (GHR), thereby activating the JAK2-STAT signaling cascade. This activation stimulates insulin-like growth factor 1 (IGF-1) production in the liver while simultaneously promoting direct tissue growth effects.Besides,Adenylate Kinase 2 (AK2) has been identified as an interactor of GH, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant pig GH and recombinant rat AK2 . Briefly, biotin-linked GH were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to AK2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant pig GH and recombinant rat AK2 was shown in Figure 1, the EC50 for this effect is 0.791µg/mL.