Active Granzyme B (GZMB)

GZM-B; HLP; CTLA1; CCPI; CGL1; CSP-B; CSPB; CTSGL1; SECT; Granzyme 2; Cytotoxic T-Lymphocyte-Associated Serine Esterase 1; Fragmentin 2; Cytotoxic Serine Protease B

  • Active Granzyme B (GZMB) Packages (Simulation)
  • Active Granzyme B (GZMB) Packages (Simulation)
  • APA600Po01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Granzyme B (GZMB)

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections. It activates various caspases and cleaves proteins such as aggrecan. The activity of recombinant pig Granzyme B was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (Assay Buffer), initiated by addition 50 μL of various concentrations of GZMB (diluted by Assay Buffer) to 50 µL of 1.2 mM Substrate and DTNB mixture. The final well serves as a negative control with no GZMB, replaced with 50 μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant pig Granzyme B is >440 pmol/min/µg.

USAGE of the Active Granzyme B (GZMB)

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Granzyme B (GZMB)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Granzyme B (GZMB)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Sus scrofa; Porcine (Pig) Applications (RESEARCH USE ONLY!)
RPA600Po01 Recombinant Granzyme B (GZMB) Positive Control; Immunogen; SDS-PAGE; WB.
APA600Po01 Active Granzyme B (GZMB) Cell culture; Activity Assays.
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