Active Glutathione S Transferase Pi (GSTp)
GSTP1; GST-P1; DFN7; FAEES3; GST3
- Product No.APB090Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point7.1
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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Figure. Western Blot
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Glutathione S Transferase Pi (GSTp)
Glutathione S-transferase P (GSTP1) is a member of Glutathione S-transferases (GSTs) family which play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340nm. The reaction was performed in adding 10μL 200mM glutathione (reduced) and 10μL 100mM CDNB in 980μL 100mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50μL mixed substrates to 50μL recombinant rat GSTP1, mix gentliy, read the absorbance at 340nm for 5min. One unit activity was defined as 1 μmole of CDNB conjugate per minute per 1 mg GSTP1
USAGE of the Active Glutathione S Transferase Pi (GSTp)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Glutathione S Transferase Pi (GSTp)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Glutathione S Transferase Pi (GSTp)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| RPB090Ra01 | Recombinant Glutathione S Transferase Pi (GSTp) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB090Ra01 | Active Glutathione S Transferase Pi (GSTp) | Cell culture; Activity Assays. |
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| LMB090Ra | Multiplex Assay Kit for Glutathione S Transferase Pi (GSTp) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
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