Active Glucosidase Alpha, Neutral AB (GaNAB)
GluII; G2AN; Alpha-glucosidase 2; Glucosidase II subunit alpha
- Product No.UAPD188Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point6.2
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 126
For more details, please contact local distributors!US$ 315
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For more details, please contact local distributors! US$ 4725
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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WB Image
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Glucosidase Alpha, Neutral AB (GaNAB)
Glucosidase Alpha, Neutral AB (GaNAB) is an enzyme in the glycoside hydrolase family. It hydrolyzes alpha - glucosidic bonds, participates in carbohydrate metabolism, and is involved in endoplasmic reticulum quality control. GaNAB binds to Heat Shock 70kDa Protein 5 (HSPA5), collaborating to ensure proper glycoprotein folding and maintain cellular protein homeostasis.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GaNAB and recombinant mouse HSPA5 .Briefly, biotin-linked GaNAB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to HSPA5-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of GaNAB and HSPA5 was shown in Figure 1, the EC50 for this effect is 3.21ug/mL.
USAGE of the Active Glucosidase Alpha, Neutral AB (GaNAB)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Glucosidase Alpha, Neutral AB (GaNAB)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Glucosidase Alpha, Neutral AB (GaNAB)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| UAPD188Hu01 | Active Glucosidase Alpha, Neutral AB (GaNAB) | Cell culture; Activity Assays. |
| URPD188Hu01 | Recombinant Glucosidase Alpha, Neutral AB (GaNAB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAD188Hu01 | Polyclonal Antibody to Glucosidase Alpha, Neutral AB (GaNAB) | WB; IHC; ICC; IP. |