Active Galectin 4 (GAL4)
LGALS4; Lactose-Binding Lectin 4; L-36 Lactose-Binding Protein; L36LBP; Lectin,Galactoside-Binding Soluble 4
- Product No.APA304Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point9.0
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Galectin 4 (GAL4)
Galectin-4(GAL4) is a protein that in humans is encoded by the LGALS4 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS4 is an S-type lectin that is strongly underexpressed in colorectal cancer. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant human GAL4 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL4 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL4, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL4 is 3.187μg/mL.
Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.
USAGE of the Active Galectin 4 (GAL4)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Galectin 4 (GAL4)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Galectin 4 (GAL4)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPA304Hu01 | Recombinant Galectin 4 (GAL4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA304Hu01 | Active Galectin 4 (GAL4) | Cell culture; Activity Assays. |
| PAA304Hu01 | Polyclonal Antibody to Galectin 4 (GAL4) | WB; IHC; ICC; IP. |
| LAA304Hu71 | Biotin-Linked Polyclonal Antibody to Galectin 4 (GAL4) | WB; IHC; ICC. |
| LAA304Hu81 | FITC-Linked Polyclonal Antibody to Galectin 4 (GAL4) | WB; IHC; ICC; IF. |
| MAA304Hu22 | Monoclonal Antibody to Galectin 4 (GAL4) | WB; IHC; ICC; IP. |
| SEA304Hu | ELISA Kit for Galectin 4 (GAL4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA304Hu | Multiplex Assay Kit for Galectin 4 (GAL4) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |