Active Galectin 2 (GAL2)

LGALS2; HL14; Lectin,Galactoside-Binding Soluble 2; Beta-galactoside-binding lectin L-14-II; Lactose-binding lectin 2; S-Lac lectin 2

  • Active Galectin 2 (GAL2) Packages (Simulation)
  • Active Galectin 2 (GAL2) Packages (Simulation)
  • APA302Po01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Galectin 2 (GAL2)

Galectin 2 (GAL2) belongs to the proto type group and consists of two homologous carbohydrate recognition domains (CRDs) resulting in multiple sugar binding sites. The expression of gal-2 has been shown to be involved in processes of angiogenesis and inflammation but was not analyzed before in preeclamptic (PE) placentas.It also can agglutinate red blood. In this case, chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant pig GAL2 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL2 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL2, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL2 is 2.5μg/ml.

Figure 2. The hemagglutination assay of GAL2 in V- bottom shaped 96-well microtiter plate

USAGE of the Active Galectin 2 (GAL2)

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Galectin 2 (GAL2)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Galectin 2 (GAL2)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Organism species: Sus scrofa; Porcine (Pig) Applications (RESEARCH USE ONLY!)
APA302Po01 Active Galectin 2 (GAL2) Cell culture; Activity Assays.
RPA302Po01 Recombinant Galectin 2 (GAL2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA302Po01 Polyclonal Antibody to Galectin 2 (GAL2) WB; IHC; ICC; IP.
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