Fibroblast Growth Factor-18 (FGF18) is a trophic factor for mature chondrocytes
and their progenitors. It has been reported to have significant anabolic effects on
cartilage. FGF18 plays a central role in skeletal growth and
development.Besides,Fibroblast Growth Factor Receptor 3 (FGFR3) has been
identified as an interactor of FGF18, thus a binding ELISA assay was conducted
to detect the interaction of recombinant human FGF18 and recombinant human
FGFR3. Briefly, FGF18 were diluted serially in PBS, with 0.01% BSA (pH 7.4).
Duplicate samples of 100μl were then transferred to FGFR3-coated microtiter
wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated
for 1h with anti-FGF18 pAb, then aspirated and washed 3 times. After incubation
with HRP labelled secondary antibody, wells were aspirated and washed 3 times.
With the addition of substrate solution, wells were incubated 15-25 minutes at
37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately.
The binding activity of FGF18 and FGFR3 was shown in Figure 1, and this effect
was in a dose dependent manner.