Erythropoietin (EPO), also known as hematopoietin or hemopoietin, is a glycoprotein cytokine secreted by the kidney in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. Erythropoietin is an essential hormone for red blood cell production. EPO can cooperate with various other growth factors involved in the development of erythroid lineage from multipotent progenitors. To test the effect of EPO on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 including various concentrations of recombinant human EPO. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of TF-1 cells after incubation with EPO for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant EPO for 72h. The result was shown in Figure 2. It was obvious that EPO significantly increased cell viability of TF-1 cells.
(A) TF-1 cells cultured in 1640, stimulated with 5ng/mL EPO for 72h;
(B) Unstimulated TF-1 cells cultured in1640 for 72h.
Figure. Cell proliferation of TF-1 cells after stimulated with EPO.

Figure. Cell proliferation of TF-1 cells after stimulated with EPO