Active Complement Component 5 (C5)
CPAMD4; C5a anaphylatoxin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
- Product No.UAPB969Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point6.4
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 130
For more details, please contact local distributors!US$ 325
For more details, please contact local distributors! US$ 650
For more details, please contact local distributors! US$ 1950
For more details, please contact local distributors! US$ 4875
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Complement Component 5 (C5)
Complement Component 5 (C5), which forms part of the later stages of the complement pathway, is cleaved into C5a and C5b. C5a is a chemotactic agent for neutrophils, while C5b forms part of the complement membrane attack complex. Coagulation Factor II (F2) has been identified as an interactor of C5, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C5 and recombinant mouse F2. Briefly, C5 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to F2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-C5 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C5 and recombinant mouse F2 was shown in Figure 1, the EC50 for this effect is 0.22 ug/mL.
USAGE of the Active Complement Component 5 (C5)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Complement Component 5 (C5)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Complement Component 5 (C5)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
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| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| URPB969Hu01 | Recombinant Complement Component 5 (C5) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB969Hu02 | Active Complement Component 5 (C5) | Cell culture; Activity Assays. |
| URPB969Hu02 | Recombinant Complement Component 5 (C5) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB969Hu01 | Polyclonal Antibody to Complement Component 5 (C5) | WB; IHC |
| UPAB969Hu02 | Polyclonal Antibody to Complement Component 5 (C5) | WB; IHC; ICC; IP. |
| ULAB969Hu71 | Biotin-Linked Polyclonal Antibody to Complement Component 5 (C5) | WB; IHC; ICC. |
| UMAB969Hu22 | Monoclonal Antibody to Complement Component 5 (C5) | WB; IHC; ICC; IP. |
| UMAB969Hu21 | Monoclonal Antibody to Complement Component 5 (C5) | WB |
| USEB969Hu | ELISA Kit for Complement Component 5 (C5) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB969Hu | Multiplex Assay Kit for Complement Component 5 (C5) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |