Active Catalase (CAT)
- Product No.UAPC418Hu05
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point6.9
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 81
For more details, please contact local distributors!US$ 203
For more details, please contact local distributors! US$ 405
For more details, please contact local distributors! US$ 1215
For more details, please contact local distributors! US$ 3038
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Catalase (CAT)
Catalase (CAT) is an antioxidant enzyme present in all aerobic organisms. It is known to catalyze H2O2 into water and oxygen in an energy-efficient manner in the cells exposed to environmental stress. As we know, H2O2 and (NH4)2MoO4 can produce a stable yellow product which has a absorbance at 405 nm. Thus, the recombinant human CAT activity was measured by its ability to hydrolyze H2O2 to H2O , the remaining H2O2 in the reaction was measured by (NH4)2MoO4. The reaction was performed in 60 mM phosphate buffer pH 7.4 (Assay Buffer), initiated by addition 100 μL of various concentrations of CAT (diluted by Assay Buffer) to 1 mL of 65 mM Substrate H2O2 (diluted by Assay Buffer). Incubated at 37℃ for 1min, then add 1 ml 200 mM (NH4)2MoO4. After 10 minutes at room temperature, read at a wavelength of 405 nm.
USAGE of the Active Catalase (CAT)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Catalase (CAT)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Catalase (CAT)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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