Active Carboxylesterase 3 (CES3)

Br3; ES31; Brain carboxylesterase 3; Esterase 31; Liver carboxylesterase 31 homolog

  • Active Carboxylesterase 3 (CES3) Packages (Simulation)
  • Active Carboxylesterase 3 (CES3) Packages (Simulation)
  • APF283Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Carboxylesterase 3 (CES3)

Carboxylesterase 3 (CES3) also known as Liver carboxylesterase 3 is a serine esterase and a member of a large family of carboxylesterases. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant mouse CES3 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, pH 7.5( Assay Buffer), initiated by addition 50 μL of various concentrations of CES3 (dilute by Assay Buffer) to 50 µL of 2 mM Substrate 4-NPA(100 mM stock in Acetone, dilute by deionized water). Incubated at 37℃ for 10min, then read at a wavelength of 400 nm.

One unit of enzyme activity is defined as the 1μg of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant mouse CES3 is 900 pmol/min/µg.

USAGE of the Active Carboxylesterase 3 (CES3)

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Carboxylesterase 3 (CES3)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Carboxylesterase 3 (CES3)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPF283Mu01 Recombinant Carboxylesterase 3 (CES3) Positive Control; Immunogen; SDS-PAGE; WB.
APF283Mu01 Active Carboxylesterase 3 (CES3) Cell culture; Activity Assays.
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