Active Carboxylesterase 1 (CES1)
ACAT; CEH; CES2; HMSE1; PCE1; SES1; TGH; Retinyl ester hydrolase; Serine esterase 1; Triacylglycerol hydrolase; Egasyn; Cocaine carboxylesterase; Liver Carboxylesterase 1
- Product No.APC374Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point6.5
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
-
FOB
US$ 336
For more details, please contact local distributors!US$ 840
For more details, please contact local distributors! US$ 1680
For more details, please contact local distributors! US$ 5040
For more details, please contact local distributors! US$ 12600
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
Figure. SDS-PAGE
-
ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Carboxylesterase 1 (CES1)
carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, pH 7.5( Assay Buffer), initiated by addition 50 μL of various concentrations of CES1 (dilute by Assay Buffer) to 50 µL of 2 mM Substrate 4-NPA(100 mM stock in Acetone, dilute by deionized water). Incubated at 37℃ for 10min, then read at a wavelength of 400 nm.
USAGE of the Active Carboxylesterase 1 (CES1)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Carboxylesterase 1 (CES1)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Carboxylesterase 1 (CES1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
BCA Protein Quantification Kit
Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Buffer
Lentivirus Packaging Experiment Service
Adenovirus Packaging Experiment Service
Real Time PCR Experimental Service
Spike RBD Protein (S-RBD)
Protein G
Protein A
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| EPC374Hu61 | Eukaryotic Carboxylesterase 1 (CES1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC374Hu01 | Active Carboxylesterase 1 (CES1) | Cell culture; Activity Assays. |
| RPC374Hu01 | Recombinant Carboxylesterase 1 (CES1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC374Hu61 | Active Carboxylesterase 1 (CES1) | Cell culture; Activity Assays. |
| PAC374Hu01 | Polyclonal Antibody to Carboxylesterase 1 (CES1) | WB; IHC; ICC; IP. |
| SEC374Hu | ELISA Kit for Carboxylesterase 1 (CES1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC374Hu | Multiplex Assay Kit for Carboxylesterase 1 (CES1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |