Active Carbonic Anhydrase XIII (CA13)

CA-XIII; CAXIII; Carbonate dehydratase XIII

  • Active Carbonic Anhydrase XIII (CA13) Packages (Simulation)
  • Active Carbonic Anhydrase XIII (CA13) Packages (Simulation)
  • APD081Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Carbonic Anhydrase XIII (CA13)

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2  H2O = HCO3-  H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA13 is a cytosolic enzyme with a unique and widespread distribution pattern as compared to the other cytosolic CAs, indicating that it may play important physiological roles in several organs. The activity of recombinant mouse CA13 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA13 (diluted by assay buffer) to 50 µL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.

USAGE of the Active Carbonic Anhydrase XIII (CA13)

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Carbonic Anhydrase XIII (CA13)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Carbonic Anhydrase XIII (CA13)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPD081Mu01 Recombinant Carbonic Anhydrase XIII (CA13) Positive Control; Immunogen; SDS-PAGE; WB.
APD081Mu01 Active Carbonic Anhydrase XIII (CA13) Cell culture; Activity Assays.
PAD081Mu01 Polyclonal Antibody to Carbonic Anhydrase XIII (CA13) WB; IHC; ICC; IP.
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