Active Carbonic Anhydrase IV (CA4)
CA-IV; CAIV; Car4; RP17; Retinitis Pigmentosa 17(Autosomal Dominant); Carbonate dehydratase IV
- Product No.APD071Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point6.4
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Carbonic Anhydrase IV (CA4)
One unit of enzyme activity is defined as the 1 μg of enzyme required to convert 1 pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant rat CA4 is > 200 pmol/min/µg.
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA4 is a GPI anchored membrane enzyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and of proximal renal tubules. It functions as the principal CO2 taste sensor. The activity of recombinant rat CA4 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of CA4 (diluted by assay buffer) to 50 µL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.
USAGE of the Active Carbonic Anhydrase IV (CA4)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Carbonic Anhydrase IV (CA4)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Carbonic Anhydrase IV (CA4)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| APD071Ra01 | Active Carbonic Anhydrase IV (CA4) | Cell culture; Activity Assays. |
| RPD071Ra01 | Recombinant Carbonic Anhydrase IV (CA4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD071Ra01 | Polyclonal Antibody to Carbonic Anhydrase IV (CA4) | WB; IHC; ICC/IF |
| MAD071Ra21 | Monoclonal Antibody to Carbonic Anhydrase IV (CA4) | WB |
| MAD071Ra24 | Monoclonal Antibody to Carbonic Anhydrase IV (CA4) | WB; IHC; ICC; IP. |
| MAD071Ra22 | Monoclonal Antibody to Carbonic Anhydrase IV (CA4) | WB; IHC; ICC; IP. |
| MAD071Ra23 | Monoclonal Antibody to Carbonic Anhydrase IV (CA4) | WB |
| MAD071Ra25 | Monoclonal Antibody to Carbonic Anhydrase IV (CA4) | WB; IHC; ICC; IP. |
| MAD071Ra26 | Monoclonal Antibody to Carbonic Anhydrase IV (CA4) | WB; IHC; ICC; IP. |