Active Calpain 2 (CAPN2)
CANPL2; CANPml; mCANP; Calpain-2 Catalytic Subunit; Calcium-activated neutral proteinase 2; Calpain M-type; Calpain large polypeptide L2; Millimolar-calpain; Calpain 2, Large Subunit
- Product No.UAPC358Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point5.0
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 114
For more details, please contact local distributors!US$ 285
For more details, please contact local distributors! US$ 570
For more details, please contact local distributors! US$ 1710
For more details, please contact local distributors! US$ 4275
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Calpain 2 (CAPN2)
Calpain 2 (CAPN2) is a calcium-regulated, non-lysosomal thiolprotease that is known for its crucial role in catalyzing the limited proteolysis of substrates essential for cytoskeletal remodeling and signaling pathways. It belongs to the family of calpains, a group of enzymes that are activated by calcium ions.Besides,CAPN1 has been identified as an interactor of CAPN2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CAPN2 and recombinant rat CAPN1. Briefly, CAPN2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CAPN1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CAPN2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant humanCAPN2 and recombinant rat CAPN1 was shown in Figure 1, the EC50 for this effect is 0.044ug/mL.
USAGE of the Active Calpain 2 (CAPN2)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Calpain 2 (CAPN2)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Calpain 2 (CAPN2)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| URPC358Hu01 | Recombinant Calpain 2 (CAPN2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC358Hu01 | Active Calpain 2 (CAPN2) | Cell culture; Activity Assays. |
| UPAC358Hu01 | Polyclonal Antibody to Calpain 2 (CAPN2) | WB; IHC; ICC/IF |
| UMAC358Hu21 | Monoclonal Antibody to Calpain 2 (CAPN2) | WB; IHC; ICC; IP. |
| USEC358Hu | ELISA Kit for Calpain 2 (CAPN2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC358Hu | Multiplex Assay Kit for Calpain 2 (CAPN2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |