Active Bone Sialoprotein (BSP)
IBSP; Bsp; BspII; SpII; Integrin-Binding Bone Sialoprotein; Cell-binding sialoprotein; Bone sialoprotein 2
- Product No.UAPB092Hu62
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point4.0
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 144
For more details, please contact local distributors!US$ 360
For more details, please contact local distributors! US$ 720
For more details, please contact local distributors! US$ 2160
For more details, please contact local distributors! US$ 5400
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Bone Sialoprotein (BSP)
Bone Sialoprotein (BSP) is a highly glycosylated and phosphorylated non-collagenous protein that plays a pivotal role in mineralized tissues, particularly in bone and dentin. As a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family, it is primarily synthesized by osteoblasts, odontoblasts, and chondrocytes, and is prominently localized in the extracellular matrix of mineralizing tissues. Additionally, it also has the function of cell adhesion. Therefore, we measure the activity of recombinant human BSP by the ability of the immobilized protein to support the adhesion of MCF-7 cell. When 5 x 104 cells/well are added to different concentrations of recombinant human BSP coated plates, cells will adhere after 2 hour incubation at 37 ℃. The adhesion of MCF-7 after 2 hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.44 μg/ml.
USAGE of the Active Bone Sialoprotein (BSP)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Bone Sialoprotein (BSP)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Bone Sialoprotein (BSP)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| UAPB092Hu61 | Active Bone Sialoprotein (BSP) | Cell culture; Activity Assays. |
| UAPB092Hu62 | Active Bone Sialoprotein (BSP) | Cell culture; Activity Assays. |
| UEPB092Hu62 | Eukaryotic Bone Sialoprotein (BSP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB092Hu01 | Recombinant Bone Sialoprotein (BSP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPB092Hu61 | Eukaryotic Bone Sialoprotein (BSP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB092Hu01 | Polyclonal Antibody to Bone Sialoprotein (BSP) | WB; IHC; ICC; IP. |
| UMAB092Hu22 | Monoclonal Antibody to Bone Sialoprotein (BSP) | WB; IHC; ICC; IP. |
| USEB092Hu | ELISA Kit for Bone Sialoprotein (BSP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB092Hu | Multiplex Assay Kit for Bone Sialoprotein (BSP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |