Active Arginase (ARG)
ARG1; Arginase I; Liver Arginase
- Product No.APB120Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point6.7
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Gene sequencing
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Figure. SDS-PAGE
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WB Image
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Arginase (ARG)
Arginase (ARG) is a kind of inducible manganese (Mn2 )-containing enzymes that catalyse the final step of the urea cycle in the liver, converting l -arginine into l -ornithine and urea.Therefore, we can measure its bioactivity by enzyme activity assay. Recombinant human Arg was diluted to different concentrations in deionized water.Loading 40 µL of the rhARG1 and 10ul Substrate mixture(100 mM Arginine, 500 mM Glycine, 1.25 mM MnCl2, pH 10.5 ) into the plate. Incubated at 37 °C for 2 hours and stop the reaction with 200 µL stop solution of the 2 mM oPA, 2 mM NED ,50 mM Boric Acid, 1 M Sulfuric Acid, 0.03% Brij-35 (w/v) .Cover the plate and incubate at room temperature for 20 minutes and read at 520 nm (absorbance) in endpoint mode. The specific activity of recombinant human ARG is >24400pmol/min/ug.
USAGE of the Active Arginase (ARG)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Arginase (ARG)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Arginase (ARG)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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