Active Adiponectin (ADPN)
GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
- Product No.APA605Ga01
- Organism SpeciesChicken (Gallus) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point6.4
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
-
FOB
US$ 260
For more details, please contact local distributors!US$ 650
For more details, please contact local distributors! US$ 1300
For more details, please contact local distributors! US$ 3900
For more details, please contact local distributors! US$ 9750
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
Figure. SDS-PAGE
-
ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Adiponectin (ADPN)
Human Adiponectin (ADPN) is a 53 kDa member of the PNPLA family of phospholipase A2 enzymes. It is a plasma protein secreted by adipose tissue showing pleiotropic effects with anti-diabetic, anti-atherogenic and anti-inflammatory properties. Adiponectin and AdipoR1 regulate PPARgC1a and mitochondria by Ca(2 ) and AMPK/SIRT1. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant chicken ADPN and recombinant rat PPARgC1a. Briefly, ADPN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PPARgC1a-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ADPN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant chicken ADPN and recombinant rat PPARgC1a was shown in Figure 1, the EC50 for this effect is 0.12 ug/mL.
USAGE of the Active Adiponectin (ADPN)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Adiponectin (ADPN)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Adiponectin (ADPN)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
BCA Protein Quantification Kit
Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Buffer
Lentivirus Packaging Experiment Service
Adenovirus Packaging Experiment Service
Real Time PCR Experimental Service
Spike RBD Protein (S-RBD)
Protein G
Protein A
Related products
| Catalog No. | Organism species: Chicken (Gallus) | Applications (RESEARCH USE ONLY!) |
| RPA605Ga01 | Recombinant Adiponectin (ADPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA605Ga01 | Active Adiponectin (ADPN) | Cell culture; Activity Assays. |
| PAA605Ga01 | Polyclonal Antibody to Adiponectin (ADPN) | WB; IHC; ICC; IP. |
| MAA605Ga21 | Monoclonal Antibody to Adiponectin (ADPN) | WB; IHC; ICC; IP. |
| SEA605Ga | ELISA Kit for Adiponectin (ADPN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA605Ga | CLIA Kit for Adiponectin (ADPN) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA605Ga | Multiplex Assay Kit for Adiponectin (ADPN) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |