Active Acid Sphingomyelinase (ASM)
SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1,Acid Lysosomal; Simply Sphingomyelinase
- Product No.UAPB360Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point7.2
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Acid Sphingomyelinase (ASM)
Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), belongs to the sphingomyelin phosphodiesterase family. The protein Converts sphingomyelin to ceramide. ASM also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.
Thus, the recombinant human ASM activity was measured by its ability to hydrolyze 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HDA-PC) to p-Nitrophenol. The reaction was performed in 50 mM MES, 0.5 µM ZnCl2, pH 7.5 ( Assay Buffer), initiated by addition 50 μL of various concentrations of ASM (diluted with Assay Buffer) to 50 µL of 1 mM Substrate HDA-PC ( 50 mM stock solution in methanol, diluted with Assay Buffer). Incubated at room temperature for 20 minutes in the dark, then read at a wavelength of 405 nm.
One unit of enzyme activity is defined as the 1μg of enzyme required to convert 1pmol of HDA-PC to p-Nitrophenol in 1min at 37°C. The specific activity of recombinant human ASM is >1000 pmol/min/µg.
USAGE of the Active Acid Sphingomyelinase (ASM)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Acid Sphingomyelinase (ASM)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Acid Sphingomyelinase (ASM)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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