Active Acid Phosphatase 1 (ACP1)
HAAP; Low Molecular Weight Phosphotyrosine Protein Phosphatase; Adipocyte acid phosphatase; Red cell acid phosphatase 1; LMW-PTPase
- Product No.APA994Mu02
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point6.7
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 176
For more details, please contact local distributors!US$ 440
For more details, please contact local distributors! US$ 880
For more details, please contact local distributors! US$ 2640
For more details, please contact local distributors! US$ 6600
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Acid Phosphatase 1 (ACP1)
Acid phosphatase locus 1 (ACP1) is a low molecular weight protein tyrosine phosphatase that has been shown to be an important regulator of insulin receptor signaling. The activity assay of recombinant mouse ACP1 was measured by its ability to cleave a peptide substrate, 4-Nitrophenyl phosphate disodium (PNPP). The reaction was performed in 50 mM NaOAc, pH 5.0 ( assay buffer), initiated by addition 50 μl of 3 ug/ml ACP1 (diluted by assay buffer) to 50 µL of 2 mM substrate. Incubated at room temperature for 5 minutes in the dark and add 100 μl of 0.2 M NaOH to stop the reaction. Read at a wavelength of 405 nm (top read), the specific activity of recombinant mouse ACP1 is >32000 pmol/min/µg.
USAGE of the Active Acid Phosphatase 1 (ACP1)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Acid Phosphatase 1 (ACP1)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Acid Phosphatase 1 (ACP1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA994Mu01 | Recombinant Acid Phosphatase 1 (ACP1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA994Mu02 | Recombinant Acid Phosphatase 1 (ACP1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA994Mu02 | Active Acid Phosphatase 1 (ACP1) | Cell culture; Activity Assays. |