Active 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3)
SDR12C2; EDH17B3; Short Chain Dehydrogenase/Reductase Family 12C,Member 2; Testosterone 17-Beta-Dehydrogenase 3; Testicular 17-beta-hydroxysteroid dehydrogenase
- Product No.UAPF173Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point8.9
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
-
FOB
US$ 133
For more details, please contact local distributors!US$ 333
For more details, please contact local distributors! US$ 666
For more details, please contact local distributors! US$ 1998
For more details, please contact local distributors! US$ 4995
For more details, please contact local distributors!
ACTIVITY TEST of the Active 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3)

17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) belongs to the HSD17B family with NAD(P)H/NAD(P) -dependent oxidoreductase activity that catalyzes the interconversion between 17-ketosteroids and 17-hydroxysteroids to maintain the balance between less potent (17-keto) and more potent (17β-hydroxy) forms of estrogens and androgens. This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5a2 ) is a enzyme which plays a central role in sexual differentiation and androgen physiology. A functional ELISA assay was conducted to detect the interaction of recombinant human HSD17b3 and recombinant human SRD5a2. Briefly, HSD17b3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to SRD5a2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HSD17b3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human HSD17b3 and recombinant human SRD5a2 was shown in Figure 1, the EC50 for this effect is 27.6 ng/mL.
USAGE of the Active 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
BCA Protein Quantification Kit
Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Buffer
Lentivirus Packaging Experiment Service
Adenovirus Packaging Experiment Service
Real Time PCR Experimental Service
Spike RBD Protein (S-RBD)
Protein G
Protein A
Related products
Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
URPF173Hu01 | Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) | Positive Control; Immunogen; SDS-PAGE; WB. |
UAPF173Hu01 | Active 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) | Cell culture; Activity Assays. |
UPAF173Hu01 | Polyclonal Antibody to 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) | WB; IHC; ICC; IP. |
UMAF173Hu21 | Monoclonal Antibody to 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) | WB; IHC; ICC; IP. |
USEF173Hu | ELISA Kit for 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
ULMF173Hu | Multiplex Assay Kit for 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |