Recombinant Antibody to Green Fluorescent Protein (GFP)
- Product No.RAD025Ge71
- Organism SpeciesPan-species (General) Same name, Different species.
- SourceRecombinant monoclonal antibody preparation
- HostCHO
- Potency (Clone Number)n/a
- Ig Isotype IgG
- PurificationProtein A + Protein G affinity chromatography
- LabelBiotin
- Immunogen EPD025Ge51-Eukaryotic Green Fluorescent Protein (GFP)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species MorePan-species (General)
- ApplicationsWB; IF; ICC; IHC; IP; FCM.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download n/a
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Briefly, biotin labelled GFP Nano-antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to GFP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed 3 times with PBST and incubated for 0.5 h at 37℃ with SA labelled HRP. After incubation with SA labelled HRP, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of GFP and GFP Nano-antibody was shown in Figure 1, and the ED50 for this effect is 0.0187 ug/ml.
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GFP was immunoprecipitated 293F whole cell extract (Expressing GFP) with 2 µg of RAD025Ge71 and 20 µl of Nickel pillar magnetic beads ( ).
Western blot was perfomed on the immunoprecipitate using 0.05µg/ml MAD025Ge21 and 0.2µg/mL SAA544Mu19.
Lane1 (input): 293F whole cell lysate.
Lane2 ( ): RAD025Ge71 in 293F whole cell lysate.
Lane3 (-): No antibody was added to the 293F whole cell lysate.
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Recombinant Antibody to Green Fluorescent Protein (GFP)
The antibody is a mouse monoclonal antibody raised against GFP. It has been selected for its ability to recognize GFP in immunohistochemical staining and western blotting.
USAGE of the Recombinant Antibody to Green Fluorescent Protein (GFP)
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Recombinant Antibody to Green Fluorescent Protein (GFP)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Recombinant Antibody to Green Fluorescent Protein (GFP)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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