Rat Model for Acute Pyelonephritis (AP)

Pyelitis; Nephritis

• Product No.UDSI559Ra01
• Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
• • All
  • Human
  • Mouse
  • Rat
  • Cavia
  • Rabbit
  • Simian
  • Caprine
  • Ovine
  • Equine
  • Bovine
  • Porcine
  • Gallus
  • Canine
  • Others
  • Multi-species
  • Pan-species
• Prototype SpeciesHuman
• Sourceinduced by bacterial infections
• Model Animal StrainsSD Rats(SPF), healthy, male and female, body weight 180g~200g.
• Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
• Modeling Period4-6 weeks
• Modeling MethodRats weight is 200 g, prohibit drinking water after 24 h. After anesthesia, the animal was supine and fixed on the operating table, abdominal skin was disinfected, sterile surgical towels were spread, and a 2cm incision was made in the middle of the lower abdomen. The abdominal wall was cut layer by layer into the abdominal cavity, and the right ureter was identified on the right posterior abdominal wall.4 suture silk thread was used to thread the needle from both sides of the right middle ureter to the lateral side of the posterior abdominal wall and lead the suture line. The penis was clipped with an artery clamp, and 0.5mL EScherichia coli（Escherichia coli，ATCC 25922） liquid of 600 nm = 0.35 ~ 0.40 was slowly injected into the bladder with TB needle.Then, the ends of the suture line outside the abdominal wall were tightened to make the ureter shrink to 1/3, and the suture line was pulled to the back of the rat to tie the knot. The incision in the abdominal wall was sutured layer by layer to restore drinking water and food supply. 24h after the operation, the ligature line outside the abdominal wall was removed and the ureter was reopened.
  
  The animals were killed at a predetermined time, both kidneys were taken, and the renal capsule was carefully removed. Then, the kidney was cut longitudinally, half of the kidney was homogenized for bacterial culture, and the other half was fixed in fixative solution, routine tissue section, HE staining, and light microscope observation. Urine bacterial culture was performed by bladder puncture at the same time.
• ApplicationsDisease Model
• Download n/a
• UOM Each case
• FOB US$ 130
  For more details, please contact local distributors!