Polyclonal Antibody to Retinoic Acid Receptor Alpha (RARa)
RAR-A; NR1B1; RAR; RAR-Alpha; Nuclear Receptor Subfamily 1 Group B Member 1
- Product No.PAA976Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA976Mu01-Recombinant Retinoic Acid Receptor Alpha (RARa)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreHomo sapiens (Human), Mus musculus (Mouse)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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Packages (Simulation)
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Packages (Simulation)
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IHC Images
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AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Mouse RARa Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11) -
AF488 staining on IF;
Sample: MCF7 cell
Primary Ab: 20µg/ml Rabbit Anti-Mouse RARa Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant RARa, Mouse.
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Figure. Western Blot; Sample: Lane1: Human A431 Cells; Lane2: Human HepG2 Cells.
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HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-mouse RARa Polyclonal Antibody (Catalog # PAA976Mu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Retinoic Acid Receptor Alpha (RARa)
The antibody is a rabbit polyclonal antibody raised against RARa. It has been selected for its ability to recognize RARa in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Retinoic Acid Receptor Alpha (RARa)
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Retinoic Acid Receptor Alpha (RARa)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Retinoic Acid Receptor Alpha (RARa)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA976Mu01 | Recombinant Retinoic Acid Receptor Alpha (RARa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA976Mu01 | Polyclonal Antibody to Retinoic Acid Receptor Alpha (RARa) | WB; IHC; ICC; IP. |
| FAA976Mu01 | Anti-Retinoic Acid Receptor Alpha (RARa) Polyclonal Antibody | Flow cytometry. |