Polyclonal Antibody to Nestin (NES)
- Product No.PAA500Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA500Mu01-Recombinant Nestin (NES)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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IHC Images
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Figure. Western Blot; Sample: Recombinant NES, Mouse.
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Mouse BALB/c 3T3 cells were fixed with 1% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-mouse NES Polyclonal Antibody (Catalog PAA500Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Nestin (NES)
The antibody is a rabbit polyclonal antibody raised against NES. It has been selected for its ability to recognize NES in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Nestin (NES)
Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Nestin (NES)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Nestin (NES)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA500Mu02 | Recombinant Nestin (NES) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA500Mu01 | Recombinant Nestin (NES) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA500Mu02 | Polyclonal Antibody to Nestin (NES) | WB; IHC; ICC; IP. |
| PAA500Mu01 | Polyclonal Antibody to Nestin (NES) | WB; IHC; ICC; IP. |
| SEA500Mu | ELISA Kit for Nestin (NES) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA500Mu | Multiplex Assay Kit for Nestin (NES) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |