Mouse Model for Sjogren Syndrome (SS)
Sjögren's syndrome; SjS
- Product No.DSI574Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Prototype SpeciesHuman
- SourceInduced by submandibular gland
- Model Animal StrainsBalb/c Mice(SPF), healthy, male, age: 4~6weeks, body weight:18g~20g.
- Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group(low,medium,high).
- Modeling Period4-6 weeks
- Modeling MethodSelect the weight of 20 g, age of 8 weeks BALB/c mice, foot pad and back subcutaneous respectively in mice multi-point injection preparation in advance (in the same rat super clean bench, conventional surgical method to ta
ke out the submandibular gland, remove coated and connective tissue, with 0.5 g/L sodium azide saline cleaning the submandibular gland, with microscopic camber of crushed into the physiological saline, then in high speed mill, general submandibular gland homogenate, maintain 4 degrees, in ultrasonic crusher broken 1 h, put such as centrifuge (3 000 r/min, the centrifugal 10 mm), take the supernatant, determine the content of protein antigens. Antigen concentration dilution: the supernatant fluid with 0.1 mol/L PBS dilute liquid into 100 mg/L, the submandibular gland antigen and volume of the CFA blending, after the formation of water-in-oil solvent concentration of 200 mg/L) of the same rat submandibular gland tissues and cells mixed antigen (submandibular gland protein) 0.5 ml, pertussis vaccine in his back.Model mice were fed on no special pathogen under the condition of constant temperature, automatic water bottles of water.In the building schedule anesthesia to be put to death animals, removing submandibular gland tissue in 4% paraformaldehyde solution, fixed out after 6 h, add 20% sucrose solution at 4 degrees in the refrigerator overnight, the glands with frozen section machine cut into slices, HE dye, were observed under light. - Applicationsn/a
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- UOM Each case
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FOB
US$ 134
For more details, please contact local distributors!
Model Evaluation of the Mouse Model for Sjogren Syndrome (SS)
Pathological Results of the Mouse Model for Sjogren Syndrome (SS)
Cytokines Level of the Mouse Model for Sjogren Syndrome (SS)
Statistical Analysis of the Mouse Model for Sjogren Syndrome (SS)
SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±s), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.
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Tissue/Sections Customized Service
Serums Customized Service
Immunohistochemistry (IHC) Experiment Service
Small Animal In Vivo Imaging Experiment Service
Small Animal Micro CT Imaging Experiment Service
Small Animal MRI Imaging Experiment Service
Small Animal Ultrasound Imaging Experiment Service
Transmission Electron Microscopy (TEM) Experiment Service
Scanning Electron Microscope (SEM) Experiment Service
Learning and Memory Behavioral Experiment Service
Anxiety and Depression Behavioral Experiment Service
Drug Addiction Behavioral Experiment Service
Pain Behavioral Experiment Service
Neuropsychiatric Disorder Behavioral Experiment Service
Fatigue Behavioral Experiment Service
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DSI574Mu01 | Mouse Model for Sjogren Syndrome (SS) | n/a |
DSI574Mu02 | Mouse Model for Sjogren Syndrome (SS) | n/a |
DSI574Mu03 | Mouse Model for Sjogren Syndrome (SS) | n/a |