Mini Samples ELISA Kit for Thyroxine (T4)

3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine

  • Mini Samples ELISA Kit for Thyroxine (T4) Packages (Simulation)
  • Mini Samples ELISA Kit for Thyroxine (T4) Packages (Simulation)
  • Mini Samples ELISA Kit for Thyroxine (T4) Results demonstration
  • MEA452Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the Mini Samples ELISA Kit for Thyroxine (T4)

This assay has high sensitivity and excellent specificity for detection of Mini Samples Thyroxine (T4).
No significant cross-reactivity or interference between Mini Samples Thyroxine (T4) and analogues was observed.

Recovery of the Mini Samples ELISA Kit for Thyroxine (T4)

Matrices listed below were spiked with certain level of Mini Samples Thyroxine (T4) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Thyroxine (T4) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-99 93
EDTA plasma(n=5) 78-90 86
heparin plasma(n=5) 98-105 102

Precision of the Mini Samples ELISA Kit for Thyroxine (T4)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Thyroxine (T4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Thyroxine (T4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the Mini Samples ELISA Kit for Thyroxine (T4)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Thyroxine (T4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-95% 82-103% 83-97% 87-96%
EDTA plasma(n=5) 95-104% 84-97% 78-99% 95-104%
heparin plasma(n=5) 94-103% 92-99% 97-105% 92-101%

Stability of the Mini Samples ELISA Kit for Thyroxine (T4)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the Mini Samples ELISA Kit for Thyroxine (T4)

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
    And then add 25μL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

Test principle of the Mini Samples ELISA Kit for Thyroxine (T4)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Thyroxine (T4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Thyroxine (T4) and unlabeled Mini Samples Thyroxine (T4) (Standards or samples) with the pre-coated antibody specific to Mini Samples Thyroxine (T4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Thyroxine (T4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Thyroxine (T4) in the sample.

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