Mini Samples ELISA Kit for Resistin (RETN)

ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3

  • Mini Samples ELISA Kit for Resistin (RETN) Packages (Simulation)
  • Mini Samples ELISA Kit for Resistin (RETN) Packages (Simulation)
  • Mini Samples ELISA Kit for Resistin (RETN) Results demonstration
  • MEA847Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the Mini Samples ELISA Kit for Resistin (RETN)

This assay has high sensitivity and excellent specificity for detection of Mini Samples Resistin (RETN).
No significant cross-reactivity or interference between Mini Samples Resistin (RETN) and analogues was observed.

Recovery of the Mini Samples ELISA Kit for Resistin (RETN)

Matrices listed below were spiked with certain level of recombinant Mini Samples Resistin (RETN) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Resistin (RETN) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-98 93
EDTA plasma(n=5) 78-97 89
heparin plasma(n=5) 79-92 87

Precision of the Mini Samples ELISA Kit for Resistin (RETN)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Resistin (RETN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Resistin (RETN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the Mini Samples ELISA Kit for Resistin (RETN)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Resistin (RETN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-97% 96-104% 96-105% 96-103%
EDTA plasma(n=5) 93-105% 92-101% 98-105% 97-104%
heparin plasma(n=5) 82-96% 83-93% 96-103% 79-95%

Stability of the Mini Samples ELISA Kit for Resistin (RETN)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the Mini Samples ELISA Kit for Resistin (RETN)

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

Test principle of the Mini Samples ELISA Kit for Resistin (RETN)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Resistin (RETN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Resistin (RETN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Resistin (RETN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Resistin (RETN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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