Mini Samples ELISA Kit for Osteocalcin (OC)

BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein

  • Mini Samples ELISA Kit for Osteocalcin (OC) Packages (Simulation)
  • Mini Samples ELISA Kit for Osteocalcin (OC) Packages (Simulation)
  • Mini Samples ELISA Kit for Osteocalcin (OC) Results demonstration
  • MEA471Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the Mini Samples ELISA Kit for Osteocalcin (OC)

This assay has high sensitivity and excellent specificity for detection of Mini Samples Osteocalcin (OC).
No significant cross-reactivity or interference between Mini Samples Osteocalcin (OC) and analogues was observed.

Recovery of the Mini Samples ELISA Kit for Osteocalcin (OC)

Matrices listed below were spiked with certain level of recombinant Mini Samples Osteocalcin (OC) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Osteocalcin (OC) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-94 91
EDTA plasma(n=5) 85-99 88
heparin plasma(n=5) 91-98 94

Precision of the Mini Samples ELISA Kit for Osteocalcin (OC)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Osteocalcin (OC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Osteocalcin (OC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the Mini Samples ELISA Kit for Osteocalcin (OC)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Osteocalcin (OC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-102% 89-96% 83-94% 90-101%
EDTA plasma(n=5) 80-101% 97-105% 81-101% 96-103%
heparin plasma(n=5) 84-99% 96-105% 80-98% 94-105%

Stability of the Mini Samples ELISA Kit for Osteocalcin (OC)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the Mini Samples ELISA Kit for Osteocalcin (OC)

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

Test principle of the Mini Samples ELISA Kit for Osteocalcin (OC)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Osteocalcin (OC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Osteocalcin (OC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Osteocalcin (OC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Osteocalcin (OC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPA471Mu02 Recombinant Osteocalcin (OC) Positive Control; Immunogen; SDS-PAGE; WB.
APA471Mu01 Active Osteocalcin (OC) Cell culture; Activity Assays.
RPA471Mu01 Recombinant Osteocalcin (OC) Positive Control; Immunogen; SDS-PAGE; WB.
PAA471Mu02 Polyclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
PAA471Mu01 Polyclonal Antibody to Osteocalcin (OC) WB; IHC; ICC; IP.
MEA471Mu Mini Samples ELISA Kit for Osteocalcin (OC) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA471Mu Instant ELISA Kit for Osteocalcin (OC) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA471Mu ELISA Kit for Osteocalcin (OC) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA471Mu01 ELISA Kit DIY Materials for Osteocalcin (OC) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK