Mini Samples ELISA Kit for Kisspeptin 1 (KISS1)
Metastin; KiSS-1 Metastasis-Suppressor
- Product No.MEC559Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.81pg/mL.
- Sample TypeSerum, plasma and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
-
FOB
US$ 605
For more details, please contact local distributors!US$ 864
For more details, please contact local distributors! US$ 3888
For more details, please contact local distributors! US$ 7344
For more details, please contact local distributors! US$ 60480
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
Results demonstration
-
Typical Standard Curve
-
ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Mini Samples ELISA Kit for Kisspeptin 1 (KISS1)
This assay has high sensitivity and excellent specificity for detection of Mini Samples Kisspeptin 1 (KISS1).
No significant cross-reactivity or interference between Mini Samples Kisspeptin 1 (KISS1) and analogues was observed.
Recovery of the Mini Samples ELISA Kit for Kisspeptin 1 (KISS1)
Matrices listed below were spiked with certain level of recombinant Mini Samples Kisspeptin 1 (KISS1) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Kisspeptin 1 (KISS1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 78-98 | 87 |
| heparin plasma(n=5) | 88-95 | 91 |
Precision of the Mini Samples ELISA Kit for Kisspeptin 1 (KISS1)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Kisspeptin 1 (KISS1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Kisspeptin 1 (KISS1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the Mini Samples ELISA Kit for Kisspeptin 1 (KISS1)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Kisspeptin 1 (KISS1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-96% | 90-101% | 87-99% | 84-99% |
| EDTA plasma(n=5) | 93-101% | 82-96% | 96-105% | 99-105% |
| heparin plasma(n=5) | 82-101% | 85-92% | 95-103% | 96-103% |
Stability of the Mini Samples ELISA Kit for Kisspeptin 1 (KISS1)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Mini Samples ELISA Kit for Kisspeptin 1 (KISS1)
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
And then add 25μL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.
Test principle of the Mini Samples ELISA Kit for Kisspeptin 1 (KISS1)
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Kisspeptin 1 (KISS1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Kisspeptin 1 (KISS1) and unlabeled Mini Samples Kisspeptin 1 (KISS1) (Standards or samples) with the pre-coated antibody specific to Mini Samples Kisspeptin 1 (KISS1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Kisspeptin 1 (KISS1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Kisspeptin 1 (KISS1) in the sample.
GIVEAWAYS
INCREMENT SERVICES
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPC559Mu01 | Recombinant Kisspeptin 1 (KISS1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC559Mu02 | Recombinant Kisspeptin 1 (KISS1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC559Mu01 | Polyclonal Antibody to Kisspeptin 1 (KISS1) | WB; IHC; ICC; IP. |
| PAC559Mu02 | Polyclonal Antibody to Kisspeptin 1 (KISS1) | WB; IHC; ICC; IP. |
| CEC559Mu | ELISA Kit for Kisspeptin 1 (KISS1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEC559Mu | Mini Samples ELISA Kit for Kisspeptin 1 (KISS1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC559Mu | Multiplex Assay Kit for Kisspeptin 1 (KISS1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |