Mini Samples ELISA Kit for Corticotropin Releasing Factor (CRF)
CRH; Corticotropin Releasing Hormone; Corticoliberin
- Product No.MEA835Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.18pg/mL.
- Sample Typeserum, plasma and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Mini Samples ELISA Kit for Corticotropin Releasing Factor (CRF)
This assay has high sensitivity and excellent specificity for detection of Mini Samples Corticotropin Releasing Factor (CRF).
No significant cross-reactivity or interference between Mini Samples Corticotropin Releasing Factor (CRF) and analogues was observed.
Recovery of the Mini Samples ELISA Kit for Corticotropin Releasing Factor (CRF)
Matrices listed below were spiked with certain level of recombinant Mini Samples Corticotropin Releasing Factor (CRF) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Corticotropin Releasing Factor (CRF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-95 | 88 |
| EDTA plasma(n=5) | 94-103 | 97 |
| heparin plasma(n=5) | 98-105 | 102 |
Precision of the Mini Samples ELISA Kit for Corticotropin Releasing Factor (CRF)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Corticotropin Releasing Factor (CRF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Corticotropin Releasing Factor (CRF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the Mini Samples ELISA Kit for Corticotropin Releasing Factor (CRF)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Corticotropin Releasing Factor (CRF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 84-98% | 79-96% | 79-95% |
| EDTA plasma(n=5) | 91-101% | 98-105% | 80-91% | 88-98% |
| heparin plasma(n=5) | 78-94% | 88-101% | 92-105% | 83-103% |
Stability of the Mini Samples ELISA Kit for Corticotropin Releasing Factor (CRF)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Mini Samples ELISA Kit for Corticotropin Releasing Factor (CRF)
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
And then add 25μL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.
Test principle of the Mini Samples ELISA Kit for Corticotropin Releasing Factor (CRF)
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Corticotropin Releasing Factor (CRF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Corticotropin Releasing Factor (CRF) and unlabeled Mini Samples Corticotropin Releasing Factor (CRF) (Standards or samples) with the pre-coated antibody specific to Mini Samples Corticotropin Releasing Factor (CRF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Corticotropin Releasing Factor (CRF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Corticotropin Releasing Factor (CRF) in the sample.