ELISA Kit for Von Willebrand Factor (vWF)

F8VWF; VWD; von Willebrand antigen 2

  • ELISA Kit for Von Willebrand Factor (vWF) Packages (Simulation)
  • ELISA Kit for Von Willebrand Factor (vWF) Packages (Simulation)
  • ELISA Kit for Von Willebrand Factor (vWF) Results demonstration
  • CEA833Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Von Willebrand Factor (vWF)

This assay has high sensitivity and excellent specificity for detection of Von Willebrand Factor (vWF).
No significant cross-reactivity or interference between Von Willebrand Factor (vWF) and analogues was observed.

Recovery of the ELISA Kit for Von Willebrand Factor (vWF)

Matrices listed below were spiked with certain level of recombinant Von Willebrand Factor (vWF) and the recovery rates were calculated by comparing the measured value to the expected amount of Von Willebrand Factor (vWF) in samples.

Matrix Recovery range (%) Average(%)
sodium citrate plasma(n=5) 79-95 86

Precision of the ELISA Kit for Von Willebrand Factor (vWF)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Von Willebrand Factor (vWF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Von Willebrand Factor (vWF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Von Willebrand Factor (vWF)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Von Willebrand Factor (vWF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 90-99% 87-95% 81-101% 89-97%

Stability of the ELISA Kit for Von Willebrand Factor (vWF)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Von Willebrand Factor (vWF)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Von Willebrand Factor (vWF)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Von Willebrand Factor (vWF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Von Willebrand Factor (vWF) and unlabeled Von Willebrand Factor (vWF) (Standards or samples) with the pre-coated antibody specific to Von Willebrand Factor (vWF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample.

Related products

Catalog No. Organism species: Rattus norvegicus (Rat) Applications (RESEARCH USE ONLY!)
RPA833Ra02 Recombinant Von Willebrand Factor (vWF) Positive Control; Immunogen; SDS-PAGE; WB.
RPA833Ra01 Recombinant Von Willebrand Factor (vWF) Positive Control; Immunogen; SDS-PAGE; WB.
CPA833Ra21 OVA Conjugated Von Willebrand Factor (vWF) Immunogen; SDS-PAGE; WB.
PAA833Ra01 Polyclonal Antibody to Von Willebrand Factor (vWF) WB; IHC; ICC; IP.
PAA833Ra02 Polyclonal Antibody to Von Willebrand Factor (vWF) WB; IHC; ICC; IP.
PAA833Ra08 Polyclonal Antibody to Von Willebrand Factor (vWF) WB; IHC; ICC; IP.
LAA833Ra71 Biotin-Linked Polyclonal Antibody to Von Willebrand Factor (vWF) WB; IHC; ICC.
LAA833Ra81 FITC-Linked Polyclonal Antibody to Von Willebrand Factor (vWF) WB; IHC; ICC; IF.
MAA833Ra21 Monoclonal Antibody to Von Willebrand Factor (vWF) WB; IHC; ICC; IP.
SEA833Ra ELISA Kit for Von Willebrand Factor (vWF) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA833Ra ELISA Kit for Von Willebrand Factor (vWF) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA833Ra11 ELISA Kit DIY Materials for Von Willebrand Factor (vWF) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK