ELISA Kit for Teduglutide (TFA)

  • ELISA Kit for Teduglutide (TFA) Packages (Simulation)
  • ELISA Kit for Teduglutide (TFA) Packages (Simulation)
  • ELISA Kit for Teduglutide (TFA) Results demonstration
  • CEX304Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Teduglutide (TFA)

This assay has high sensitivity and excellent specificity for detection of Teduglutide (TFA).
No significant cross-reactivity or interference between Teduglutide (TFA) and analogues was observed.

Recovery of the ELISA Kit for Teduglutide (TFA)

Matrices listed below were spiked with certain level of Teduglutide (TFA) and the recovery rates were calculated by comparing the measured value to the expected amount of Teduglutide (TFA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-101 91
EDTA plasma(n=5) 82-99 88
heparin plasma(n=5) 98-105 101

Precision of the ELISA Kit for Teduglutide (TFA)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Teduglutide (TFA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Teduglutide (TFA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Teduglutide (TFA)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Teduglutide (TFA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-93% 84-102% 85-93% 89-101%
EDTA plasma(n=5) 84-96% 87-95% 83-93% 80-90%
heparin plasma(n=5) 81-92% 80-92% 78-97% 79-92%

Stability of the ELISA Kit for Teduglutide (TFA)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Teduglutide (TFA)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Teduglutide (TFA)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Teduglutide (TFA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Teduglutide (TFA) and unlabeled Teduglutide (TFA) (Standards or samples) with the pre-coated antibody specific to Teduglutide (TFA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Teduglutide (TFA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Teduglutide (TFA) in the sample.

Related products

Catalog No. Organism species: Pan-species (General) Applications (RESEARCH USE ONLY!)
CEX304Ge ELISA Kit for Teduglutide (TFA) Enzyme-linked immunosorbent assay for Antigen Detection.
AEX304Ge ELISA Kit for Anti-Teduglutide Antibody (Anti-TFA) Enzyme-linked immunosorbent assay for Antibody Detection.
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