ELISA Kit for Matrix Metalloproteinase 9 (MMP9)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Rattus norvegicus (Rat)
Product No.SEA553Ra
Sample typeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Format96-well strip plate
Assay length4.5 hours
Detection range2.34-150ng/mL The standard curve concentrations used for the ELISA’s were 150ng/mL, 75ng/mL, 37.5ng/mL, 18.75ng/mL, 9.38ng/mL, 4.69ng/mL, 2.34ng/mL
SensitivityThe minimum detectable dose of this kit is typically less than 0.9ng/mL.
Specificity
This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 9 (MMP9).
No significant cross-reactivity or interference between Matrix Metalloproteinase 9 (MMP9) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 9 (MMP9) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 9 (MMP9) in samples.
Matrix Recovery range (%) Average(%)
serum(n=5)97-105102
heparin plasma(n=5)90-10295
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 9 (MMP9) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
serum(n=5)94-101%95-103%85-94%92-104%
heparin plasma(n=5)85-101%87-96%97-105%86-93%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 9 (MMP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 9 (MMP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 9 (MMP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 9 (MMP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Package and Components
Reagent Preparation
Results demonstration
Typical Standard Curve
Certificate
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Free use and Guarantees
Free trial
In case it is difficult to pre-evaluate experiment result because of specific test samples or other reasons, free trial can be offered.
If the budget is really limited or other special situation, you can apply the free products.
Guarantees
Unconditionally return policy within 72 hours.
For any complaint, we promise to reply within one work day, resolve the problem within three work days. Implementing initial consultation system in order to provide satisfactory service for all the customers. Customers can unconditionally to request a charge back or refund.
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MagazineReference
Journal of Surgical Research Serum Levels of Matrix Metalloproteinase-2 as a Marker of Intimal Hyperplasia    Read more(ScienceDirect: S0022480409001942)
Journal of Proteome Research The Biological Behaviors of Rat Dermal Fibroblasts Can Be Inhibited by High Levels of MMP9    Read more(ACS: pr2006395)
Experimental & Translational Stroke Medicine Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke    Read more(PubMed: 21679435)
Rheumatology International Hypothermia induced by adenosine 5′-monophosphate attenuates early stage injury in an acute gouty arthritis rat model    Read more(Pubmed: 23408150)
Clinical and Investigative Medicine The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells    Read more(Pubmed: 23544611)
International journal of colorectal disease Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12 in early colonic anastomotic repair    Read more(Pubmed: 23619615)
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications
MAA553Ra21Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
PAA553Ra01Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
PAA553Ra02Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
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RPA553Ra51Recombinant Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP.
SEA553RaELISA Kit for Matrix Metalloproteinase 9 (MMP9)Enzyme-linked immunosorbent assay