ELISA Kit for Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA)

  • ELISA Kit for Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) Packages (Simulation)
  • ELISA Kit for Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) Packages (Simulation)
  • ELISA Kit for Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) Results demonstration
  • AEX292Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA)

This assay has high sensitivity and excellent specificity for detection of Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA).
No significant cross-reactivity or interference between Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) and analogues was observed.

Recovery of the ELISA Kit for Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA)

Matrices listed below were spiked with certain level of Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) and the recovery rates were calculated by comparing the measured value to the expected amount of Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-105 101
EDTA plasma(n=5) 79-98 82
heparin plasma(n=5) 79-89 84

Precision of the ELISA Kit for Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-104% 93-105% 78-96% 92-99%
EDTA plasma(n=5) 90-99% 79-96% 80-93% 80-91%
heparin plasma(n=5) 85-92% 96-103% 88-95% 78-95%

Stability of the ELISA Kit for Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA)

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Perinuclear Anti-Neutrophil Cytoplasmic Antibody (pANCA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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