ELISA Kit for Sequestosome 1 (SQSTM1)

OSIL; PDB3; ZIP3; p62; p62B; p60; A170; Paget Disease Of Bone 3; Oxidative Stress Induced Like; EBI3-associated protein of 60 kDa; Ubiquitin-binding protein p62

  • ELISA Kit for Sequestosome 1 (SQSTM1) Packages (Simulation)
  • ELISA Kit for Sequestosome 1 (SQSTM1) Packages (Simulation)
  • ELISA Kit for Sequestosome 1 (SQSTM1) Results demonstration
  • SED198Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Sequestosome 1 (SQSTM1)

This assay has high sensitivity and excellent specificity for detection of Sequestosome 1 (SQSTM1).
No significant cross-reactivity or interference between Sequestosome 1 (SQSTM1) and analogues was observed.

Precision of the ELISA Kit for Sequestosome 1 (SQSTM1)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sequestosome 1 (SQSTM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sequestosome 1 (SQSTM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability of the ELISA Kit for Sequestosome 1 (SQSTM1)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Sequestosome 1 (SQSTM1)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Sequestosome 1 (SQSTM1)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sequestosome 1 (SQSTM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sequestosome 1 (SQSTM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sequestosome 1 (SQSTM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sequestosome 1 (SQSTM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPD198Hu01 Recombinant Sequestosome 1 (SQSTM1) Positive Control; Immunogen; SDS-PAGE; WB.
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MAD198Hu21 Monoclonal Antibody to Sequestosome 1 (SQSTM1) WB; IHC; ICC; IP.
SED198Hu ELISA Kit for Sequestosome 1 (SQSTM1) Enzyme-linked immunosorbent assay for Antigen Detection.
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