ELISA Kit for Laminin Beta 3 (LAMb3)
LAM-B3; LAMNB1; Nicein subunit beta; Kalinin subunit beta; Epiligrin subunit bata; Laminin B1k chain; Laminin-5 subunit beta
- Product No.SEC081Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.78-50ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.33ng/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors!US$ 700
For more details, please contact local distributors! US$ 3150
For more details, please contact local distributors! US$ 5950
For more details, please contact local distributors! US$ 49000
For more details, please contact local distributors!
- Packages (Simulation)
- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Laminin Beta 3 (LAMb3)
This assay has high sensitivity and excellent specificity for detection of Laminin Beta 3 (LAMb3).
No significant cross-reactivity or interference between Laminin Beta 3 (LAMb3) and analogues was observed.
Recovery of the ELISA Kit for Laminin Beta 3 (LAMb3)
Matrices listed below were spiked with certain level of recombinant Laminin Beta 3 (LAMb3) and the recovery rates were calculated by comparing the measured value to the expected amount of Laminin Beta 3 (LAMb3) in samples.
|Matrix||Recovery range (%)||Average(%)|
Precision of the ELISA Kit for Laminin Beta 3 (LAMb3)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin Beta 3 (LAMb3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin Beta 3 (LAMb3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Linearity of the ELISA Kit for Laminin Beta 3 (LAMb3)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Laminin Beta 3 (LAMb3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Stability of the ELISA Kit for Laminin Beta 3 (LAMb3)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Laminin Beta 3 (LAMb3)
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the ELISA Kit for Laminin Beta 3 (LAMb3)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Beta 3 (LAMb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Beta 3 (LAMb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Beta 3 (LAMb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Beta 3 (LAMb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Single-component Reagents of Assay Kit
Lysis Buffer Specific for ELISA / CLIA
Quality Control of ELISA / CLIA
ELISA Kit Customized Service
Disease Model Customized Service
Serums Customized Service
ELISA / CLIA Experiment Service
TGFB1 Activation Reagent
Real Time PCR Experimental Service
Glutathione S-Transferase (GSH-ST) Assay Kit
Microscale Reduced Glutathione assay kit
Glutathione Reductase Activity Coefficient (GRAC) Assay Kit
Angiotensin Converting Enzyme Kit
|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|RPC081Hu01||Recombinant Laminin Beta 3 (LAMb3)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAC081Hu01||Polyclonal Antibody to Laminin Beta 3 (LAMb3)||WB; IHC; ICC; IP.|
|MAC081Hu22||Monoclonal Antibody to Laminin Beta 3 (LAMb3)||WB; IHC; ICC; IP.|
|SEC081Hu||ELISA Kit for Laminin Beta 3 (LAMb3)||Enzyme-linked immunosorbent assay for Antigen Detection.|