ELISA Kit for Heparan Sulfate Proteoglycan (HSPG)

SDC2; HSPG1; SYND2; Fibroglycan; Syndecan 2; Syndecan Proteoglycan 2; Heparan Sulfate Proteoglycan 1,Cell Surface-Associated; Heparan Sulphate Proteoglycan

  • ELISA Kit for Heparan Sulfate Proteoglycan (HSPG) Packages (Simulation)
  • ELISA Kit for Heparan Sulfate Proteoglycan (HSPG) Packages (Simulation)
  • ELISA Kit for Heparan Sulfate Proteoglycan (HSPG) Results demonstration
  • SEA565Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Heparan Sulfate Proteoglycan (HSPG)

This assay has high sensitivity and excellent specificity for detection of Heparan Sulfate Proteoglycan (HSPG).
No significant cross-reactivity or interference between Heparan Sulfate Proteoglycan (HSPG) and analogues was observed.

Recovery of the ELISA Kit for Heparan Sulfate Proteoglycan (HSPG)

Matrices listed below were spiked with certain level of recombinant Heparan Sulfate Proteoglycan (HSPG) and the recovery rates were calculated by comparing the measured value to the expected amount of Heparan Sulfate Proteoglycan (HSPG) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-105 101
EDTA plasma(n=5) 84-103 96
heparin plasma(n=5) 84-94 90

Precision of the ELISA Kit for Heparan Sulfate Proteoglycan (HSPG)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparan Sulfate Proteoglycan (HSPG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparan Sulfate Proteoglycan (HSPG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Heparan Sulfate Proteoglycan (HSPG)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heparan Sulfate Proteoglycan (HSPG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-101% 90-99% 79-101% 97-105%
EDTA plasma(n=5) 80-88% 78-95% 91-104% 79-104%
heparin plasma(n=5) 78-101% 89-98% 94-101% 82-98%

Stability of the ELISA Kit for Heparan Sulfate Proteoglycan (HSPG)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Heparan Sulfate Proteoglycan (HSPG)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Heparan Sulfate Proteoglycan (HSPG)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate Proteoglycan (HSPG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparan Sulfate Proteoglycan (HSPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate Proteoglycan (HSPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparan Sulfate Proteoglycan (HSPG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPA565Hu01 Recombinant Heparan Sulfate Proteoglycan (HSPG) Positive Control; Immunogen; SDS-PAGE; WB.
PAA565Hu01 Polyclonal Antibody to Heparan Sulfate Proteoglycan (HSPG) WB; IHC; ICC; IP.
LAA565Hu71 Biotin-Linked Polyclonal Antibody to Heparan Sulfate Proteoglycan (HSPG) WB; IHC; ICC.
LAA565Hu81 FITC-Linked Polyclonal Antibody to Heparan Sulfate Proteoglycan (HSPG) WB; IHC; ICC; IF.
MAA565Hu22 Monoclonal Antibody to Heparan Sulfate Proteoglycan (HSPG) WB; IHC; ICC; IP.
SEA565Hu ELISA Kit for Heparan Sulfate Proteoglycan (HSPG) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA565Hu01 ELISA Kit DIY Materials for Heparan Sulfate Proteoglycan (HSPG) Main materials for "Do It(ELISA Kit) Yourself"
GUESTBOOK