ELISA Kit for Immunoglobulin G (IgG)

  • ELISA Kit for Immunoglobulin G (IgG) Packages (Simulation)
  • ELISA Kit for Immunoglobulin G (IgG) Packages (Simulation)
  • ELISA Kit for Immunoglobulin G (IgG) Results demonstration
  • CEA544Gu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Immunoglobulin G (IgG)

This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G (IgG).
No significant cross-reactivity or interference between Immunoglobulin G (IgG) and analogues was observed.

Recovery of the ELISA Kit for Immunoglobulin G (IgG)

Matrices listed below were spiked with certain level of recombinant Immunoglobulin G (IgG) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin G (IgG) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-101 95
EDTA plasma(n=5) 90-102 97
heparin plasma(n=5) 84-91 87

Precision of the ELISA Kit for Immunoglobulin G (IgG)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Immunoglobulin G (IgG)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G (IgG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-96% 80-98% 86-105% 89-101%
EDTA plasma(n=5) 86-99% 95-102% 86-95% 93-101%
heparin plasma(n=5) 84-101% 92-101% 78-96% 87-104%

Stability of the ELISA Kit for Immunoglobulin G (IgG)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Immunoglobulin G (IgG)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 5 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Immunoglobulin G (IgG)

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Immunoglobulin G (IgG) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G (IgG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Cavia (Guinea pig ) Applications (RESEARCH USE ONLY!)
NPA544Gu01 Native Immunoglobulin G (IgG) Positive Control; Immunogen; SDS-PAGE; WB.
PAA544Gu01 Polyclonal Antibody to Immunoglobulin G (IgG) WB; IHC; ICC; IP.
SAA544Gu09 HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody WB; IHC; ICC.
SAA544Gu07 Biotin-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody WB; IHC; ICC.
SAA544Gu08 FITC-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody WB; IHC; ICC; IF.
MAA544Gu21 Monoclonal Antibody to Immunoglobulin G (IgG) WB; IHC; ICC; IP.
CEA544Gu ELISA Kit for Immunoglobulin G (IgG) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA544Gu CLIA Kit for Immunoglobulin G (IgG) Chemiluminescent immunoassay for Antigen Detection.
KSA544Gu11 ELISA Kit DIY Materials for Immunoglobulin G (IgG) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK