ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)
CK2; CK-MB; Creatine Kinase,Muscle/Brain
- Product No.SEA479Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.60ng/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)
This assay has high sensitivity and excellent specificity for detection of Creatine Kinase MB Isoenzyme (CKMB).
No significant cross-reactivity or interference between Creatine Kinase MB Isoenzyme (CKMB) and analogues was observed.
Recovery of the ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)
Matrices listed below were spiked with certain level of recombinant Creatine Kinase MB Isoenzyme (CKMB) and the recovery rates were calculated by comparing the measured value to the expected amount of Creatine Kinase MB Isoenzyme (CKMB) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 95-104 | 101 |
| EDTA plasma(n=5) | 91-99 | 96 |
| heparin plasma(n=5) | 86-101 | 94 |
Precision of the ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Creatine Kinase MB Isoenzyme (CKMB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Creatine Kinase MB Isoenzyme (CKMB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Creatine Kinase MB Isoenzyme (CKMB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-101% | 82-105% | 97-105% | 78-91% |
| EDTA plasma(n=5) | 84-102% | 80-96% | 96-105% | 89-97% |
| heparin plasma(n=5) | 96-103% | 92-101% | 94-105% | 80-95% |
Stability of the ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)
Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Construct a standard curve by plotting the mean O.D. and concentration for each standard and draw a best fit curve through the points on the graph or create a standard curve on log-log graph paper with CKMB concentration on the y-axis and absorbance on the x-axis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
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Related products
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