ELISA Kit for Copeptin (CPP)

C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin

  • ELISA Kit for Copeptin (CPP) Packages (Simulation)
  • ELISA Kit for Copeptin (CPP) Packages (Simulation)
  • ELISA Kit for Copeptin (CPP) Results demonstration
  • SEA365Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Copeptin (CPP)

This assay has high sensitivity and excellent specificity for detection of Copeptin (CPP).
No significant cross-reactivity or interference between Copeptin (CPP) and analogues was observed.

Recovery of the ELISA Kit for Copeptin (CPP)

Matrices listed below were spiked with certain level of recombinant Copeptin (CPP) and the recovery rates were calculated by comparing the measured value to the expected amount of Copeptin (CPP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 101
EDTA plasma(n=5) 89-103 98
heparin plasma(n=5) 81-97 87

Precision of the ELISA Kit for Copeptin (CPP)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Copeptin (CPP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Copeptin (CPP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Copeptin (CPP)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Copeptin (CPP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-94% 79-101% 92-101% 81-90%
EDTA plasma(n=5) 96-105% 85-101% 80-103% 97-104%
heparin plasma(n=5) 89-96% 88-103% 87-101% 89-99%

Stability of the ELISA Kit for Copeptin (CPP)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Copeptin (CPP)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Copeptin (CPP)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Copeptin (CPP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Copeptin (CPP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Copeptin (CPP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Copeptin (CPP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPA365Hu01 Recombinant Copeptin (CPP) Positive Control; Immunogen; SDS-PAGE; WB.
CPA365Hu31 KLH Conjugated Copeptin (CPP) Immunogen; SDS-PAGE; WB.
CPA365Hu22 OVA Conjugated Copeptin (CPP) Immunogen; SDS-PAGE; WB.
CPA365Hu21 OVA Conjugated Copeptin (CPP) Immunogen; SDS-PAGE; WB.
PAA365Hu01 Polyclonal Antibody to Copeptin (CPP) WB; IHC; ICC; IP.
PAA365Hu08 Polyclonal Antibody to Copeptin (CPP) WB; IHC; ICC; IP.
MAA365Hu28 Monoclonal Antibody to Copeptin (CPP) WB; IHC; ICC; IP.
SEA365Hu ELISA Kit for Copeptin (CPP) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA365Hu High Sensitive ELISA Kit for Copeptin (CPP) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA365Hu ELISA Kit for Copeptin (CPP) Enzyme-linked immunosorbent assay for Antigen Detection.
GUESTBOOK