ELISA Kit for Brain Natriuretic Peptide (BNP)

GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32

  • ELISA Kit for Brain Natriuretic Peptide (BNP) Packages (Simulation)
  • ELISA Kit for Brain Natriuretic Peptide (BNP) Packages (Simulation)
  • ELISA Kit for Brain Natriuretic Peptide (BNP) Results demonstration
  • SEA541Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Brain Natriuretic Peptide (BNP)

This assay has high sensitivity and excellent specificity for detection of Brain Natriuretic Peptide (BNP).
No significant cross-reactivity or interference between Brain Natriuretic Peptide (BNP) and analogues was observed.

Recovery of the ELISA Kit for Brain Natriuretic Peptide (BNP)

Matrices listed below were spiked with certain level of recombinant Brain Natriuretic Peptide (BNP) and the recovery rates were calculated by comparing the measured value to the expected amount of Brain Natriuretic Peptide (BNP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-92 80
EDTA plasma(n=5) 97-105 102
heparin plasma(n=5) 82-89 86

Precision of the ELISA Kit for Brain Natriuretic Peptide (BNP)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Natriuretic Peptide (BNP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Natriuretic Peptide (BNP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Brain Natriuretic Peptide (BNP)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Brain Natriuretic Peptide (BNP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 88-95% 94-105% 87-103%
EDTA plasma(n=5) 92-101% 96-105% 85-97% 86-101%
heparin plasma(n=5) 90-104% 98-105% 93-105% 98-105%

Stability of the ELISA Kit for Brain Natriuretic Peptide (BNP)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Brain Natriuretic Peptide (BNP)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Brain Natriuretic Peptide (BNP)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Natriuretic Peptide (BNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Natriuretic Peptide (BNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Natriuretic Peptide (BNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Natriuretic Peptide (BNP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPA541Hu01 Recombinant Brain Natriuretic Peptide (BNP) Positive Control; Immunogen; SDS-PAGE; WB.
SPA541Hu01 Synthetic Brain Natriuretic Peptide (BNP) Immunogen; Blocking Peptide.
CPA541Hu21 OVA Conjugated Brain Natriuretic Peptide (BNP) Immunogen; SDS-PAGE; WB.
CPA541Hu11 BSA Conjugated Brain Natriuretic Peptide (BNP) Immunogen; SDS-PAGE; WB.
CPA541Hu22 OVA Conjugated Brain Natriuretic Peptide (BNP) Immunogen; SDS-PAGE; WB.
CPA541Hu23 OVA Conjugated Brain Natriuretic Peptide (BNP) Immunogen; SDS-PAGE; WB.
CPA541Hu24 OVA Conjugated Brain Natriuretic Peptide (BNP) Immunogen; SDS-PAGE; WB.
PAA541Hu01 Polyclonal Antibody to Brain Natriuretic Peptide (BNP) WB; IHC; ICC; IP.
PAA541Hu02 Polyclonal Antibody to Brain Natriuretic Peptide (BNP) WB; IHC; ICC; IP.
PAA541Hu07 Polyclonal Antibody to Brain Natriuretic Peptide (BNP) WB; IHC; ICC; IP.
PAA541Hu08 Polyclonal Antibody to Brain Natriuretic Peptide (BNP) WB; IHC; ICC; IP.
PAA541Hu09 Polyclonal Antibody to Brain Natriuretic Peptide (BNP) WB; IHC; ICC; IP.
LAA541Hu81 FITC-Linked Polyclonal Antibody to Brain Natriuretic Peptide (BNP) WB; IHC; ICC; IF.
LAA541Hu71 Biotin-Linked Polyclonal Antibody to Brain Natriuretic Peptide (BNP) WB; IHC; ICC.
MAA541Hu22 Monoclonal Antibody to Brain Natriuretic Peptide (BNP) WB; IHC; ICC; IP.
MAA541Hu26 Monoclonal Antibody to Brain Natriuretic Peptide (BNP) WB; IHC; ICC; IP.
MAA541Hu28 Monoclonal Antibody to Brain Natriuretic Peptide (BNP) WB; IHC; ICC; IP.
SEA541Hu ELISA Kit for Brain Natriuretic Peptide (BNP) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA541Hu ELISA Kit for Brain Natriuretic Peptide (BNP) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA541Hu CLIA Kit for Brain Natriuretic Peptide (BNP) Chemiluminescent immunoassay for Antigen Detection.
KSA541Hu11 ELISA Kit DIY Materials for Brain Natriuretic Peptide (BNP) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK