ELISA Kit for Androstenedione (ASD)
4-Androstenedione; 4-Androstene-3,17-Dione
- Product No.CEA456Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range123.5-10,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 44.2pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
-
FOB
US$ 557
For more details, please contact local distributors!US$ 796
For more details, please contact local distributors! US$ 3580
For more details, please contact local distributors! US$ 6763
For more details, please contact local distributors! US$ 55692
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
Results demonstration
-
Typical Standard Curve
-
ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Androstenedione (ASD)
This assay has high sensitivity and excellent specificity for detection of Androstenedione (ASD).
No significant cross-reactivity or interference between Androstenedione (ASD) and analogues was observed.
Recovery of the ELISA Kit for Androstenedione (ASD)
Matrices listed below were spiked with certain level of Androstenedione (ASD) and the recovery rates were calculated by comparing the measured value to the expected amount of Androstenedione (ASD) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-102 | 98 |
| EDTA plasma(n=5) | 83-90 | 86 |
| heparin plasma(n=5) | 81-95 | 90 |
Precision of the ELISA Kit for Androstenedione (ASD)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Androstenedione (ASD) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Androstenedione (ASD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the ELISA Kit for Androstenedione (ASD)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Androstenedione (ASD) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-96% | 96-104% | 96-103% | 87-101% |
| EDTA plasma(n=5) | 84-101% | 79-101% | 95-105% | 94-102% |
| heparin plasma(n=5) | 99-105% | 85-92% | 78-105% | 79-101% |
Stability of the ELISA Kit for Androstenedione (ASD)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Androstenedione (ASD)
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle of the ELISA Kit for Androstenedione (ASD)
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Androstenedione (ASD) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Androstenedione (ASD) and unlabeled Androstenedione (ASD) (Standards or samples) with the pre-coated antibody specific to Androstenedione (ASD). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Androstenedione (ASD) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Androstenedione (ASD) in the sample.
GIVEAWAYS
INCREMENT SERVICES
Related products
| Catalog No. | Organism species: Pan-species (General) | Applications (RESEARCH USE ONLY!) |
| CPA456Ge11 | BSA Conjugated Androstenedione (ASD) | Immunogen; SDS-PAGE; WB. |
| CPA456Ge21 | OVA Conjugated Androstenedione (ASD) | Immunogen; SDS-PAGE; WB. |
| PAA456Ge01 | Polyclonal Antibody to Androstenedione (ASD) | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA456Ge71 | Biotin-Linked Polyclonal Antibody to Androstenedione (ASD) | WB; IHC; ICC. |
| CEA456Ge | ELISA Kit for Androstenedione (ASD) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA456Ge | Instant ELISA Kit for Androstenedione (ASD) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA456Ge | Multiplex Assay Kit for Androstenedione (ASD) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA456Ge11 | ELISA Kit DIY Materials for Androstenedione (ASD) | Main materials for "Do It (ELISA Kit) Yourself". |