ELISA Kit DIY Materials for Osteonectin (ON)
SPARC; BM-40; Secreted Protein,Acidic,Cysteine-Rich; Basement-membrane protein 40; Secreted protein acidic and rich in cysteine
- Product No.KSA791Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range3.12-200ng/mL
- Applicable Sensitivity1.42ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Download n/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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FOB
US$ 1134
For more details, please contact local distributors!US$ 1890
For more details, please contact local distributors! US$ 3402
For more details, please contact local distributors! US$ 6615
For more details, please contact local distributors! US$ 11340
For more details, please contact local distributors!
SPECIFITY of the ELISA Kit DIY Materials for Osteonectin (ON)
The Abs in the kit have high sensitivity and excellent specificity for detection of Osteonectin (ON). No significant cross-reactivity or interference between Osteonectin (ON) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Osteonectin (ON)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Osteonectin (ON)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
SUPPORT PACK of the ELISA Kit DIY Materials for Osteonectin (ON)
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