ELISA Kit DIY Materials for Endothelin 1 (EDN1)
ET1; PPET1; Preproendothelin-1; Big endothelin-1
- Product No.KSA482Ra11
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range6.17-500pg/mL
- Applicable Sensitivity2.71pg/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Download n/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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FOB
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the ELISA Kit DIY Materials for Endothelin 1 (EDN1)
The Abs in the kit have high sensitivity and excellent specificity for detection of Endothelin 1 (EDN1). No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Endothelin 1 (EDN1)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Endothelin 1 (EDN1)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
SUPPORT PACK of the ELISA Kit DIY Materials for Endothelin 1 (EDN1)
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Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| RPA482Ra01 | Recombinant Endothelin 1 (EDN1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA482Ra01 | Active Endothelin 1 (EDN1) | Cell culture; Activity Assays. |
| CPA482Ra11 | BSA Conjugated Endothelin 1 (EDN1) | Immunogen; SDS-PAGE; WB. |
| CPA482Ra21 | OVA Conjugated Endothelin 1 (EDN1) | Immunogen; SDS-PAGE; WB. |
| PAA482Ra08 | Polyclonal Antibody to Endothelin 1 (EDN1) | WB; IHC; ICC; IP. |
| PAA482Ra01 | Polyclonal Antibody to Endothelin 1 (EDN1) | WB; IHC; ICC; IP. |
| LAA482Ra81 | FITC-Linked Polyclonal Antibody to Endothelin 1 (EDN1) | WB; IHC; ICC; IF. |
| LAA482Ra71 | Biotin-Linked Polyclonal Antibody to Endothelin 1 (EDN1) | WB; IHC; ICC. |
| MAA482Ra21 | Monoclonal Antibody to Endothelin 1 (EDN1) | WB; IHC; ICC; IP. |
| CEA482Ra | ELISA Kit for Endothelin 1 (EDN1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA482Ra | Multiplex Assay Kit for Endothelin 1 (EDN1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA482Ra11 | ELISA Kit DIY Materials for Endothelin 1 (EDN1) | Main materials for "Do It (ELISA Kit) Yourself". |