CLIA Kit for Xanthine Dehydrogenase (XDH)

XO; XOR; XOD; Xanthine Oxidase; NAD+-Xanthine Dehydrogenase; Xanthine-NAD+ Oxidoreductase; Xanthine/NAD+ Oxidoreductase; Xanthine Oxidoreductase

  • CLIA Kit for Xanthine Dehydrogenase (XDH) Packages (Simulation)
  • CLIA Kit for Xanthine Dehydrogenase (XDH) Packages (Simulation)
  • CLIA Kit for Xanthine Dehydrogenase (XDH) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Xanthine Dehydrogenase (XDH)

This assay has high sensitivity and excellent specificity for detection of Xanthine Dehydrogenase (XDH).
No significant cross-reactivity or interference between Xanthine Dehydrogenase (XDH) and analogues was observed.

Recovery of the CLIA Kit for Xanthine Dehydrogenase (XDH)

Matrices listed below were spiked with certain level of recombinant Xanthine Dehydrogenase (XDH) and the recovery rates were calculated by comparing the measured value to the expected amount of Xanthine Dehydrogenase (XDH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-99 96
EDTA plasma(n=5) 83-98 93
heparin plasma(n=5) 92-101 97

Precision of the CLIA Kit for Xanthine Dehydrogenase (XDH)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Xanthine Dehydrogenase (XDH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Xanthine Dehydrogenase (XDH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Xanthine Dehydrogenase (XDH)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Xanthine Dehydrogenase (XDH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 87-97% 87-95% 87-98%
EDTA plasma(n=5) 81-103% 89-104% 94-103% 97-104%
heparin plasma(n=5) 97-105% 90-101% 83-105% 87-94%

Stability of the CLIA Kit for Xanthine Dehydrogenase (XDH)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Xanthine Dehydrogenase (XDH)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Xanthine Dehydrogenase (XDH)

The microplate provided in this kit has been pre-coated with an antibody specific to Xanthine Dehydrogenase (XDH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Xanthine Dehydrogenase (XDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Xanthine Dehydrogenase (XDH) level in the sample or standard.;

Related products

Catalog No. Organism species: Bos taurus; Bovine (Cattle) Applications (RESEARCH USE ONLY!)
RPC608Bo01 Recombinant Xanthine Dehydrogenase (XDH) Positive Control; Immunogen; SDS-PAGE; WB.
PAC608Bo01 Polyclonal Antibody to Xanthine Dehydrogenase (XDH) WB; IHC; ICC; IP.
MAC608Bo21 Monoclonal Antibody to Xanthine Dehydrogenase (XDH) WB; IHC; ICC; IP.
SEC608Bo ELISA Kit for Xanthine Dehydrogenase (XDH) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC608Bo CLIA Kit for Xanthine Dehydrogenase (XDH) Chemiluminescent immunoassay for Antigen Detection.
LMC608Bo Multiplex Assay Kit for Xanthine Dehydrogenase (XDH) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK