CLIA Kit for Tryptase (TPS)

TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III

  • CLIA Kit for Tryptase (TPS) Packages (Simulation)
  • CLIA Kit for Tryptase (TPS) Packages (Simulation)
  • CLIA Kit for Tryptase (TPS) Results demonstration
  • SCB070Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Tryptase (TPS)

This assay has high sensitivity and excellent specificity for detection of Tryptase (TPS).
No significant cross-reactivity or interference between Tryptase (TPS) and analogues was observed.

Recovery of the CLIA Kit for Tryptase (TPS)

Matrices listed below were spiked with certain level of recombinant Tryptase (TPS) and the recovery rates were calculated by comparing the measured value to the expected amount of Tryptase (TPS) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-103 101
EDTA plasma(n=5) 87-104 94
heparin plasma(n=5) 97-105 101

Precision of the CLIA Kit for Tryptase (TPS)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tryptase (TPS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tryptase (TPS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Tryptase (TPS)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tryptase (TPS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-89% 97-105% 78-99% 95-103%
EDTA plasma(n=5) 80-99% 79-91% 78-89% 97-105%
heparin plasma(n=5) 90-98% 80-90% 96-103% 83-101%

Stability of the CLIA Kit for Tryptase (TPS)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Tryptase (TPS)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Tryptase (TPS)

The microplate provided in this kit has been pre-coated with an antibody specific to Tryptase (TPS). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tryptase (TPS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tryptase (TPS) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPB070Hu01 Recombinant Tryptase (TPS) Positive Control; Immunogen; SDS-PAGE; WB.
PAB070Hu01 Polyclonal Antibody to Tryptase (TPS) WB; IHC; ICC; IP.
LAB070Hu71 Biotin-Linked Polyclonal Antibody to Tryptase (TPS) WB; IHC; ICC.
MAB070Hu21 Monoclonal Antibody to Tryptase (TPS) WB; IHC; ICC; IP.
MAB070Hu24 Monoclonal Antibody to Tryptase (TPS) WB; IHC; ICC; IP.
MAB070Hu26 Monoclonal Antibody to Tryptase (TPS) WB; IHC; ICC; IP.
MAB070Hu22 Monoclonal Antibody to Tryptase (TPS) WB; IHC; ICC; IP.
LAB070Hu76 Biotin-Linked Monoclonal Antibody to Tryptase (TPS) WB; IHC; ICC.
WEB070Hu Wide-range ELISA Kit for Tryptase (TPS) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB070Hu ELISA Kit for Tryptase (TPS) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB070Hu CLIA Kit for Tryptase (TPS) Chemiluminescent immunoassay for Antigen Detection.
PSB070Hu01 Antibody Pair for Tryptase (TPS) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB070Hu01 ELISA Kit DIY Materials for Tryptase (TPS) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK