CLIA Kit for Cardiac Troponin I (cTnI)

TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac

  • CLIA Kit for Cardiac Troponin I (cTnI) Packages (Simulation)
  • CLIA Kit for Cardiac Troponin I (cTnI) Packages (Simulation)
  • CLIA Kit for Cardiac Troponin I (cTnI) Results demonstration
  • SCA478Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Cardiac Troponin I (cTnI)

This assay has high sensitivity and excellent specificity for detection of Cardiac Troponin I (cTnI).
No significant cross-reactivity or interference between Cardiac Troponin I (cTnI) and analogues was observed.

Recovery of the CLIA Kit for Cardiac Troponin I (cTnI)

Matrices listed below were spiked with certain level of recombinant Cardiac Troponin I (cTnI) and the recovery rates were calculated by comparing the measured value to the expected amount of Cardiac Troponin I (cTnI) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-105 90
EDTA plasma(n=5) 82-103 87
heparin plasma(n=5) 80-90 82

Precision of the CLIA Kit for Cardiac Troponin I (cTnI)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cardiac Troponin I (cTnI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cardiac Troponin I (cTnI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Cardiac Troponin I (cTnI)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cardiac Troponin I (cTnI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-101% 78-98% 89-103% 78-102%
EDTA plasma(n=5) 80-94% 84-92% 78-94% 84-95%
heparin plasma(n=5) 94-105% 86-93% 97-104% 91-101%

Stability of the CLIA Kit for Cardiac Troponin I (cTnI)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Cardiac Troponin I (cTnI)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Cardiac Troponin I (cTnI)

The microplate provided in this kit has been pre-coated with an antibody specific to Cardiac Troponin I (cTnI). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cardiac Troponin I (cTnI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cardiac Troponin I (cTnI) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPA478Hu02 Recombinant Cardiac Troponin I (cTnI) Positive Control; Immunogen; SDS-PAGE; WB.
EPA478Hu61 Eukaryotic Cardiac Troponin I (cTnI) Positive Control; Immunogen; SDS-PAGE; WB.
RPA478Hu01 Recombinant Cardiac Troponin I (cTnI) Positive Control; Immunogen; SDS-PAGE; WB.
APA478Hu01 Active Cardiac Troponin I (cTnI) Cell culture; Activity Assays.
PAA478Hu01 Polyclonal Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC; IP.
LAA478Hu71 Biotin-Linked Polyclonal Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC.
MAA478Hu28 Monoclonal Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC; IP.
RAA478Hu21 Recombinant Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC.
MAA478Hu21 Monoclonal Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC; IP.
MAA478Hu27 Monoclonal Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC; IP.
MAA478Hu23 Monoclonal Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC; IP.
MAA478Hu24 Monoclonal Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC; IP.
MAA478Hu25 Monoclonal Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC; IP.
MAA478Hu29 Monoclonal Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC; IP.
MAA478Hu22 Monoclonal Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC; IP.
MAA478Hu26 Monoclonal Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC; IP.
FAA478Hu08 Anti-Cardiac Troponin I (cTnI) Monoclonal Antibody FCM
FAA478Hu06 Anti-Cardiac Troponin I (cTnI) Monoclonal Antibody Flow cytometry.
FAA478Hu04 Anti-Cardiac Troponin I (cTnI) Monoclonal Antibody Flow cytometry.
FAA478Hu02 Anti-Cardiac Troponin I (cTnI) Monoclonal Antibody Flow cytometry.
LAA478Hu74 Biotin-Linked Monoclonal Antibody to Cardiac Troponin I (cTnI) WB; IHC; ICC.
SEA478Hu ELISA Kit for Cardiac Troponin I (cTnI) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA478Hu High Sensitive ELISA Kit for Cardiac Troponin I (cTnI) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA478Hu ELISA Kit for Anti-Cardiac Troponin I Antibody (Anti-cTnI) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA478Hu CLIA Kit for Cardiac Troponin I (cTnI) Chemiluminescent immunoassay for Antigen Detection.
LMA478Hu Multiplex Assay Kit for Cardiac Troponin I (cTnI) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA478Hu01 ELISA Kit DIY Materials for Cardiac Troponin I (cTnI) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK